Review



c1 single cell auto prep reagent kit  (fluidigm)


Bioz Verified Symbol fluidigm is a verified supplier
Bioz Manufacturer Symbol fluidigm manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    fluidigm c1 single cell auto prep reagent kit
    C1 Single Cell Auto Prep Reagent Kit, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1+single+cell+auto+prep+reagent+kit/C1+Single-Cell+Reagent+Kit+for+mRNA+Seq/pmc10202654__mmc9-519-28-37
    Average 93 stars, based on 28 article reviews
    c1 single cell auto prep reagent kit - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Lysis:

    Article Title: Hypoxia-Driven TGFβ Modulation of Side Population Cells in Breast Cancer: The Potential Role of ERα.
    Article Snippet: Cells were separated using a C1 Single-Cell Preamp integrated fluidic circuit (size 10–17 μm) (Fluidigm). .. IFC priming, cell loading and lysis, reverse transcription and pre-amplification was then carried out using a range of kits including the C1 Single-Cell Auto Prep Reagent Kit (Fluidigm), Ambion Single Cell-to-CT qRT-PCR Kit (Thermo Fisher Scientific) and 20X TaqMan Gene Expression primers (Thermo Fisher Scientific). .. After loading, partitioned Cancers 2023, 15, 1108 6 of 18 single cells were visualized under bright field and florescence using an Axiovert 200 M microscope (Zeiss).

    Article Title: A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis.
    Article Snippet: The sorted single cells (a 10:1 mixture of EGFP+E-Cadherin+CD9+KIT-/low and EGFP–E-Cadherin+CD9+KIT-/low Aundiff fractions) were captured individually on a C1 Single-Cell Auto Prep Array for PreAmp (Fluidigm) designed for 10- to 17-um cells using a Fluidigm C1 system (Fluidigm). .. Cell lysis, cDNA synthesis, and preamplification were performed using Single Cell-to-CT Kit (Thermo), C1 Single-Cell Auto Prep Reagent kit, and specific primers for arbitrarily selected target genes (Table S2). qPCR were performed on a 96:96 dynamic array chips (Fluidigm) using GE 96.96 Dynamic Array DNA Binding Dye Sample & Loading Reagent Kit (Fluidigm), and SsoFast EvaGreen Supermix with Low ROX (Bio-Rad), in a Biomark HD system (Fluidigm) platform, following the manufacturer’s instructions. .. The Raw data from 60 cells were analyzed in RStudio software (https://www.rstudio. com/).

    Reverse Transcription:

    Article Title: Hypoxia-Driven TGFβ Modulation of Side Population Cells in Breast Cancer: The Potential Role of ERα.
    Article Snippet: Cells were separated using a C1 Single-Cell Preamp integrated fluidic circuit (size 10–17 μm) (Fluidigm). .. IFC priming, cell loading and lysis, reverse transcription and pre-amplification was then carried out using a range of kits including the C1 Single-Cell Auto Prep Reagent Kit (Fluidigm), Ambion Single Cell-to-CT qRT-PCR Kit (Thermo Fisher Scientific) and 20X TaqMan Gene Expression primers (Thermo Fisher Scientific). .. After loading, partitioned Cancers 2023, 15, 1108 6 of 18 single cells were visualized under bright field and florescence using an Axiovert 200 M microscope (Zeiss).

    Article Title: Mesenchymal loss of p53 alters stem cell capacity and models human soft tissue sarcoma traits
    Article Snippet: Single-cell cDNA synthesis was performed with a C1 91 Single-Cell Auto Prep System (Fluidigm). .. Reverse transcription and preamplification were performed 92 according to the manufacturer’s instructions using a SMARTer Ultra Low RNA Kit for the Fluidigm C1 93 System (Clontech) and a C1 Single-Cell Auto Prep Reagent Kit for mRNA Seq (Fluidigm). .. For RNA controls, 94 tubes #1, #4, and #7 of ArrayControl RNA Spikes (Thermo Fisher Scientific) were mixed at a ratio of 95 100:10:1, and the RNA Spikes mixture was diluted 1:10000 in C1 Loading Reagent (Fluidigm).

    Article Title: Mesenchymal loss of p53 alters stem cell capacity and models human soft tissue sarcoma traits
    Article Snippet: Single-cell cDNA synthesis was performed with a C1 91 Single-Cell Auto Prep System (Fluidigm). .. Reverse transcription and preamplification were performed 92 according to the manufacturer’s instructions using a SMARTer Ultra Low RNA Kit for the Fluidigm C1 93 System (Clontech) and a C1 Single-Cell Auto Prep Reagent Kit for mRNA Seq (Fluidigm). .. For RNA controls, 94 tubes #1, #4, and #7 of ArrayControl RNA Spikes (Thermo Fisher Scientific) were mixed at a ratio of 95 100:10:1, and the RNA Spikes mixture was diluted 1:10000 in C1 Loading Reagent (Fluidigm).

    Single Cell:

    Article Title: Hypoxia-Driven TGFβ Modulation of Side Population Cells in Breast Cancer: The Potential Role of ERα.
    Article Snippet: Cells were separated using a C1 Single-Cell Preamp integrated fluidic circuit (size 10–17 μm) (Fluidigm). .. IFC priming, cell loading and lysis, reverse transcription and pre-amplification was then carried out using a range of kits including the C1 Single-Cell Auto Prep Reagent Kit (Fluidigm), Ambion Single Cell-to-CT qRT-PCR Kit (Thermo Fisher Scientific) and 20X TaqMan Gene Expression primers (Thermo Fisher Scientific). .. After loading, partitioned Cancers 2023, 15, 1108 6 of 18 single cells were visualized under bright field and florescence using an Axiovert 200 M microscope (Zeiss).

    Article Title: Nuclear activation in dual-durotaxing cells on a matrix with cell-scale stiffness-heterogeneity
    Article Snippet: Single-cell separation was performed using the C1 system (Fluidigm, CA, USA). .. The cell suspension was mixed with the cell suspension reagent from the C1 Single-Cell Auto Prep Reagent kit (Fluidigm) at a ratio of 3:2 (cell suspension 6 μl + cell suspension reagent 4 μl). .. Next, 6 μl of the cell mixture was loaded onto the primed Integrated Fluidic Circuit (IFC, C1 Single-Cell Auto Prep for Preamp 17-25 μm, Fluidigm), and cells were then captured into the IFC according to the manufacturer’s instructions.

    Article Title: Mesenchymal loss of p53 alters stem cell capacity and models human soft tissue sarcoma traits
    Article Snippet: Single-cell cDNA synthesis was performed with a C1 91 Single-Cell Auto Prep System (Fluidigm). .. Reverse transcription and preamplification were performed 92 according to the manufacturer’s instructions using a SMARTer Ultra Low RNA Kit for the Fluidigm C1 93 System (Clontech) and a C1 Single-Cell Auto Prep Reagent Kit for mRNA Seq (Fluidigm). .. For RNA controls, 94 tubes #1, #4, and #7 of ArrayControl RNA Spikes (Thermo Fisher Scientific) were mixed at a ratio of 95 100:10:1, and the RNA Spikes mixture was diluted 1:10000 in C1 Loading Reagent (Fluidigm).

    Article Title: A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis.
    Article Snippet: The sorted single cells (a 10:1 mixture of EGFP+E-Cadherin+CD9+KIT-/low and EGFP–E-Cadherin+CD9+KIT-/low Aundiff fractions) were captured individually on a C1 Single-Cell Auto Prep Array for PreAmp (Fluidigm) designed for 10- to 17-um cells using a Fluidigm C1 system (Fluidigm). .. Cell lysis, cDNA synthesis, and preamplification were performed using Single Cell-to-CT Kit (Thermo), C1 Single-Cell Auto Prep Reagent kit, and specific primers for arbitrarily selected target genes (Table S2). qPCR were performed on a 96:96 dynamic array chips (Fluidigm) using GE 96.96 Dynamic Array DNA Binding Dye Sample & Loading Reagent Kit (Fluidigm), and SsoFast EvaGreen Supermix with Low ROX (Bio-Rad), in a Biomark HD system (Fluidigm) platform, following the manufacturer’s instructions. .. The Raw data from 60 cells were analyzed in RStudio software (https://www.rstudio. com/).

    Article Title: Targeting 3′ and 5′ untranslated regions with antisense oligonucleotides to stabilize frataxin mRNA and increase protein expression
    Article Snippet: Single-cell analyses were performed using the Fluidigm C1 system in combination with standard qPCR method. .. In brief, 17–25-μm Fluidigm integrated fluidic circuits (Fluidigm, Cat# 100-5758) and C1 Single-Cell Auto Prep Reagent Kit (Fluidigm, Cat# 100-5319) were used to capture and lyse individual cells. .. Subsequently, reverse transcription and pre-amplification were performed using PreAmp Ambion Single Cell-to-CTTM qRT-PCR Kit (Life Technologies, Cat# 4458237).

    Article Title: Nuclear activation in dual-durotaxing cells on a matrix with cell-scale stiffness-heterogeneity
    Article Snippet: Single-cell separation was performed using the C1 system (Fluidigm, CA, USA). .. The cell suspension was mixed with the cell suspension reagent from the C1 Single-Cell Auto Prep Reagent kit (Fluidigm) at a ratio of 3:2 (cell suspension 6 μl + cell suspension reagent 4 μl). .. Next, 6 μl of the cell mixture was loaded onto the primed Integrated Fluidic Circuit (IFC, C1 Single-Cell Auto Prep for Preamp 17-25 μm, Fluidigm), and cells were then captured into the IFC according to the manufacturer’s instructions.

    Article Title: Mesenchymal loss of p53 alters stem cell capacity and models human soft tissue sarcoma traits
    Article Snippet: Single-cell cDNA synthesis was performed with a C1 91 Single-Cell Auto Prep System (Fluidigm). .. Reverse transcription and preamplification were performed 92 according to the manufacturer’s instructions using a SMARTer Ultra Low RNA Kit for the Fluidigm C1 93 System (Clontech) and a C1 Single-Cell Auto Prep Reagent Kit for mRNA Seq (Fluidigm). .. For RNA controls, 94 tubes #1, #4, and #7 of ArrayControl RNA Spikes (Thermo Fisher Scientific) were mixed at a ratio of 95 100:10:1, and the RNA Spikes mixture was diluted 1:10000 in C1 Loading Reagent (Fluidigm).

    Quantitative RT-PCR:

    Article Title: Hypoxia-Driven TGFβ Modulation of Side Population Cells in Breast Cancer: The Potential Role of ERα.
    Article Snippet: Cells were separated using a C1 Single-Cell Preamp integrated fluidic circuit (size 10–17 μm) (Fluidigm). .. IFC priming, cell loading and lysis, reverse transcription and pre-amplification was then carried out using a range of kits including the C1 Single-Cell Auto Prep Reagent Kit (Fluidigm), Ambion Single Cell-to-CT qRT-PCR Kit (Thermo Fisher Scientific) and 20X TaqMan Gene Expression primers (Thermo Fisher Scientific). .. After loading, partitioned Cancers 2023, 15, 1108 6 of 18 single cells were visualized under bright field and florescence using an Axiovert 200 M microscope (Zeiss).

    Gene Expression:

    Article Title: Hypoxia-Driven TGFβ Modulation of Side Population Cells in Breast Cancer: The Potential Role of ERα.
    Article Snippet: Cells were separated using a C1 Single-Cell Preamp integrated fluidic circuit (size 10–17 μm) (Fluidigm). .. IFC priming, cell loading and lysis, reverse transcription and pre-amplification was then carried out using a range of kits including the C1 Single-Cell Auto Prep Reagent Kit (Fluidigm), Ambion Single Cell-to-CT qRT-PCR Kit (Thermo Fisher Scientific) and 20X TaqMan Gene Expression primers (Thermo Fisher Scientific). .. After loading, partitioned Cancers 2023, 15, 1108 6 of 18 single cells were visualized under bright field and florescence using an Axiovert 200 M microscope (Zeiss).

    Suspension:

    Article Title: Nuclear activation in dual-durotaxing cells on a matrix with cell-scale stiffness-heterogeneity
    Article Snippet: Single-cell separation was performed using the C1 system (Fluidigm, CA, USA). .. The cell suspension was mixed with the cell suspension reagent from the C1 Single-Cell Auto Prep Reagent kit (Fluidigm) at a ratio of 3:2 (cell suspension 6 μl + cell suspension reagent 4 μl). .. Next, 6 μl of the cell mixture was loaded onto the primed Integrated Fluidic Circuit (IFC, C1 Single-Cell Auto Prep for Preamp 17-25 μm, Fluidigm), and cells were then captured into the IFC according to the manufacturer’s instructions.

    Article Title: Nuclear activation in dual-durotaxing cells on a matrix with cell-scale stiffness-heterogeneity
    Article Snippet: Single-cell separation was performed using the C1 system (Fluidigm, CA, USA). .. The cell suspension was mixed with the cell suspension reagent from the C1 Single-Cell Auto Prep Reagent kit (Fluidigm) at a ratio of 3:2 (cell suspension 6 μl + cell suspension reagent 4 μl). .. Next, 6 μl of the cell mixture was loaded onto the primed Integrated Fluidic Circuit (IFC, C1 Single-Cell Auto Prep for Preamp 17-25 μm, Fluidigm), and cells were then captured into the IFC according to the manufacturer’s instructions.

    cDNA Synthesis:

    Article Title: A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis.
    Article Snippet: The sorted single cells (a 10:1 mixture of EGFP+E-Cadherin+CD9+KIT-/low and EGFP–E-Cadherin+CD9+KIT-/low Aundiff fractions) were captured individually on a C1 Single-Cell Auto Prep Array for PreAmp (Fluidigm) designed for 10- to 17-um cells using a Fluidigm C1 system (Fluidigm). .. Cell lysis, cDNA synthesis, and preamplification were performed using Single Cell-to-CT Kit (Thermo), C1 Single-Cell Auto Prep Reagent kit, and specific primers for arbitrarily selected target genes (Table S2). qPCR were performed on a 96:96 dynamic array chips (Fluidigm) using GE 96.96 Dynamic Array DNA Binding Dye Sample & Loading Reagent Kit (Fluidigm), and SsoFast EvaGreen Supermix with Low ROX (Bio-Rad), in a Biomark HD system (Fluidigm) platform, following the manufacturer’s instructions. .. The Raw data from 60 cells were analyzed in RStudio software (https://www.rstudio. com/).

    Real-time Polymerase Chain Reaction:

    Article Title: A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis.
    Article Snippet: The sorted single cells (a 10:1 mixture of EGFP+E-Cadherin+CD9+KIT-/low and EGFP–E-Cadherin+CD9+KIT-/low Aundiff fractions) were captured individually on a C1 Single-Cell Auto Prep Array for PreAmp (Fluidigm) designed for 10- to 17-um cells using a Fluidigm C1 system (Fluidigm). .. Cell lysis, cDNA synthesis, and preamplification were performed using Single Cell-to-CT Kit (Thermo), C1 Single-Cell Auto Prep Reagent kit, and specific primers for arbitrarily selected target genes (Table S2). qPCR were performed on a 96:96 dynamic array chips (Fluidigm) using GE 96.96 Dynamic Array DNA Binding Dye Sample & Loading Reagent Kit (Fluidigm), and SsoFast EvaGreen Supermix with Low ROX (Bio-Rad), in a Biomark HD system (Fluidigm) platform, following the manufacturer’s instructions. .. The Raw data from 60 cells were analyzed in RStudio software (https://www.rstudio. com/).

    Binding Assay:

    Article Title: A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis.
    Article Snippet: The sorted single cells (a 10:1 mixture of EGFP+E-Cadherin+CD9+KIT-/low and EGFP–E-Cadherin+CD9+KIT-/low Aundiff fractions) were captured individually on a C1 Single-Cell Auto Prep Array for PreAmp (Fluidigm) designed for 10- to 17-um cells using a Fluidigm C1 system (Fluidigm). .. Cell lysis, cDNA synthesis, and preamplification were performed using Single Cell-to-CT Kit (Thermo), C1 Single-Cell Auto Prep Reagent kit, and specific primers for arbitrarily selected target genes (Table S2). qPCR were performed on a 96:96 dynamic array chips (Fluidigm) using GE 96.96 Dynamic Array DNA Binding Dye Sample & Loading Reagent Kit (Fluidigm), and SsoFast EvaGreen Supermix with Low ROX (Bio-Rad), in a Biomark HD system (Fluidigm) platform, following the manufacturer’s instructions. .. The Raw data from 60 cells were analyzed in RStudio software (https://www.rstudio. com/).



    Similar Products

    93
    fluidigm c1 single cell auto prep reagent kit
    C1 Single Cell Auto Prep Reagent Kit, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1+single+cell+auto+prep+reagent+kit/C1+Single-Cell+Reagent+Kit+for+mRNA+Seq/pmc10202654__mmc9-519-28-37
    Average 93 stars, based on 1 article reviews
    c1 single cell auto prep reagent kit - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    90
    fluidigm c1 single-cell auto prep reagent kit mrna seq
    C1 Single Cell Auto Prep Reagent Kit Mrna Seq, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1+single+cell+auto+prep+reagent+kit/c1+single+cell+auto+prep+reagent+kit+for+mrna+seq/pmc10202654__mmc1-45-35-37
    Average 90 stars, based on 1 article reviews
    c1 single-cell auto prep reagent kit mrna seq - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    94
    fluidigm resource source identifier c1 single cell auto prep reagent kit fluidigm
    Resource Source Identifier C1 Single Cell Auto Prep Reagent Kit Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1+single+cell+auto+prep+reagent+kit/C1+Single-Cell+Reagent+Kit+for+Preamp/pm34686326-241-2-11
    Average 94 stars, based on 1 article reviews
    resource source identifier c1 single cell auto prep reagent kit fluidigm - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results